Abstract
The use of ultraviolet absorption measurements for the estimation of RNA by the Schmidt-Thannhauser procedure has been examined on samples of rat liver. Considerable amounts of protein are released into the RNA fraction during prolonged digestion in alkali, and the consequent errors in ultraviolet absorption cannot be adequately corrected by taking readings at two wavelengths and applying equations. On the other hand, a 1-h period of digestion in alkali at 37° releases negligible quantities of protein, provided that the concentration of alkali is low. Recovery of RNA is not impaired by this shortening of the period of digestion. Lipids need not be removed from the tissue.
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