The potential regulatory effect of nitric oxide in plasma activated water on cell growth of Saccharomyces cerevisiae

Abstract

Plasma activated water (PAW) has shown a promising prospect for applications in the medical and food industries. In this study, the influence of nitric oxide radical (NO.) on the PAW regulatory capability was investigated. Electron paramagnetic resonance was employed to systematically detect the exact concentrations of NO. in PAW. It was observed that NO. concentration depended on plasma generation power, increasing with the augment of electrical parameters. Accordingly, the survival rates of S. cerevisiae were analyzed after PAW treatments, which had a negative correlation with NO. concentrations. Furthermore, the results demonstrated that NO. with low concentration in PAW had a promotive effect on cell growth, while NO. with high concentration in PAW had an inhibitory effect. It was speculated that NO. may be involved in the regulation of PAW on cell growth, which shed light on the further understanding in the interaction of PAW with biological cell.