The Potential of the Quick Detection of Selectins Using Raman Spectroscopy to Discriminate Lung Cancer Patients from Healthy Subjects

Edyta Wolny-Rokicka,Agnieszka Zembroń-Łacny,Jerzy Wydmański,Andrzej Tukiendorf

doi:10.1155/2018/7843208

Abstract

This study aimed at determining the concentration of P-selectins in lung cancer patients in different stages and healthy subjects. Then, the ability of the methodology developed to discriminate the existence of lung cancer was also evaluated. Serum spectra were obtained using Raman spectroscopy (RS). Blood samples were taken from subjects divided into two groups: group 1—comparing data from 22 patients clinically diagnosed with cancer before versus after medical intervention; group 2—comparing data from 10 palliative patients versus 17 healthy volunteers. The RS analysis of the samples revealed the presence of five very similar peaks in both groups 1 and 2. This leads to the conclusion that a medical intervention in cancer cases gives results comparable to those obtained from healthy subjects. The study indicates that the use of Raman spectroscopy can produce a better classification of cancer patients. However, diagnostically the results have not been statistically significant, probably due to the limited number of samples gathered. A larger number of samples would be required for future verification.

Highlights

P-selectin is a molecule which belongs to the selectin family together with P-platelets, E-endothelials, and L-leukocytes
It was recognized that P-selectin may be a candidate involved in the metastatic process [8,9,10]. ere are studies reporting that P-selectin plays a functional role in metastasis formation in breast, colon [11, 12], and lung cancers [10, 13]. ere was a study which explained a deeper role of P-selectin. e study showed that after the removal of the cell surface mucin from tumor cells in the lungs of mice, a reduction of metastasis can be observed [8]
It was found that some band ratios were significant and corresponded to the concentration of P-selectin in serum samples. ose specific bands might be helpful in screening for lung cancer using Raman spectroscopy of serum samples

Summary

Introduction

P-selectin is a molecule which belongs to the selectin family together with P-platelets, E-endothelials, and L-leukocytes. Ere are studies reporting that P-selectin plays a functional role in metastasis formation in breast, colon [11, 12], and lung cancers [10, 13]. P-selectin is described as a potential candidate for biomarkers whose higher concentration is presented and involved in the Journal of Spectroscopy metastatic process [8, 11, 14,15,16].

Results

Conclusion