The Potential of Sea Grapes (Caulerpa Lentilifera) Extracted Polysaccharide as Prebiotics on Inhibiting Pathogenic Bacteria Vibrio Parahaemolyticus

Anek Sopon,Ekthida Thongdet,Sucharat Suksai,Porntep Punnarak

doi:10.18178/ijesd.2020.11.12.1309

Abstract

Sea grapes or green cavier (Caulerpa lentilifera), a common tropical green seaweed, consisted of polysaccharides to be used as a prebiotic precursor for prevention of pathogens in aquatic animals. The efficiency of polysaccharides extracted from sea grapes for prebiotic properties was conducted by comparing the growth of probiotic bacteria Bacillus subtilis at different concentrations in co-cultured with pathogenic bacteria Vibrio parahaemolyticus, a gram-negative motile bacterium that inhabits marine and estuarine environments throughout the world, causes of violent diseases outbreak in aquatic animals. The experimental research was 4 treatments with 4 replications including control group (LB broth), extracted sea grape enrichment in LB broth at 0.5, 1.0 and 2.7 mg.C/l. The result showed that the highest growth of probiotic bacteria appeared significantly at extracted sea grape in LB broth with 0.5 mg.C/l (1.64×107 ± 6.04×106 cfu/ml). The said concentration was used as benchmark to clarify the pathogenic resistance. The comparison between monoculture of probiotic bacteria and co-culture of probiotic bacteria plus pathogenic bacteria indicated that there was non significantly different in growth of the bacterias. Hence extracted polysaccharides from sea graps (C. lentilifera) had potential to be utilized not only as a growth enrichment of probiotic bacteria but also inhibiting pathogenic bacteria.

Highlights

One of the important sources of polysaccharides is seaweed due to the high content aside from being a source of protein increased food nutrition for animals
Growth of B. subtilis cultured in LB broth media with 3 concentrations of polysaccharide of extracted from sea grape (0.5, 1.0, 2.7 mg.C/l) and 0 mg.C/l of extracted polysaccharide as control were compared at 0, 24, and 48 hours after incubation using total plate count technique, initial inoculation varied between 2.50×104 ± 3.79×104 to 1.55×105 ± 1.06×105 cfu/ml (Table I)
The results showed that after 24 hours of incubation, the amount of B. subtilis cultured in LB broth with 0.5 mg.C/l extracted polysaccharide was 1.64×107 ±6.04×106 cfu/ml, significantly (p < 0.05) higher than amount of B. subtilis cultured in LB broth with 1.0 and 2.7 mg.C/l (3.40×105 ± 4.98×105 cfu/ml and non-detected, respectively) and tent to higher than control (4.57×106±3.38×106 cfu/ml) as showed in Table I and Fig. 1

Summary

Introduction

One of the important sources of polysaccharides is seaweed due to the high content aside from being a source of protein increased food nutrition for animals. This work was part of the project the study on efficiency of polysaccharide from Seaweed for applied to prebiotic was supported by National Research Council of Thailand (NRCT). Both types of polysaccharide through fermentation processes can be a source of carbon for growth of all types of bacteria. Polysaccharides are considered as prebiotics and valuable in inhibiting pathogens. Polysaccharide provides carbon source for probiotic which is a group of useful bacteria. In aquaculture Bacillus sp., Lactobacillus sp. and Bifidobacterium are interested and commonly applied bacterial group

Methods

Results

Discussion

Conclusion