Abstract
The rat chimera is an important animal model for the study of complex human diseases. In the present study we evaluated the chimeric potential of rat inner cell masses (ICMs) and fetal neural stem (FNS) cells. In result, three rat chimeras were produced by day 5 (D5) Sprague-Dawley (SD) blastocysts injected with ICMs derived from day 6 (D6) and D5 Dark Agouti (DA) blastocysts; four rat chimeras had been generated by D5 DA blastocyst injected with D5 SD ICMs. For the requirement of gene modification, cultured rat inner cell mass cells were assessed to produce chimeras, but no chimeras were generated from injected embryos. The potential to generate chimeras from rFNS and transfected rFNS cells were tested, but no chimeric pups were produced. Only 2 of 41 fetuses derived from D5 DA blastocyst injection with SD LacZ transfected rFNS cells showed very low number of LacZ positive cells in the section. These results indicate that DA and SD rat ICMs are able to contribute to chimeras, but their potential decreases significantly after culture in vitro (P<0.05), and rFNS cells only have the potential to contribute to early fetal development.
Highlights
The rat chimera is an important animal model for the study of complex human diseases
The results indicated that rat inner cell masses (ICMs) derived from early blastocysts on day 5 and fully expended blastocysts on day 6 both had the potential to contribute to chimeras after blastocyst injection
The results suggested that rat ICMs freshly isolated from blastocysts had potential to develop into chimeras, but the chimeric potential of rat ICMs might be lost after culture in vitro for 6 days. 2.2 Potential development of chimeras from blastocyst injection of rat fetal neural stem cells Twenty pups were born from 148 Dark Agouti (DA) rat blastocysts injected with SD rFNS cells following embryo transfer into seven Hooded Wistar (HW) recipients
Summary
The rat chimera is an important animal model for the study of complex human diseases. 2 of 41 fetuses derived from D5 DA blastocyst injection with SD LacZ transfected rFNS cells showed very low number of LacZ positive cells in the section These results indicate that DA and SD rat ICMs are able to contribute to chimeras, but their potential decreases significantly after culture in vitro (P
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