The Potential of Polyethylene Terephthalate Glycol as Biomaterial for Bone Tissue Engineering.

Paulo Bártolo,Boyang Huang,Evangelos Daskalakis,Mohamed H. Hassan,Ilya Strashnov,Yanhao Hou,Abdalla M. Omar,Bruce D. Grieve

doi:10.3390/polym12123045

Paulo Bártolo, Boyang Huang + Show 6 more

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https://doi.org/10.3390/polym12123045

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Journal: Polymers	Publication Date: Dec 18, 2020
Citations: 33	License type: CC BY 4.0

Affiliation: University of Manchester

Abstract

The search for materials with improved mechanical and biological properties is a major challenge in tissue engineering. This paper investigates, for the first time, the use of Polyethylene Terephthalate Glycol (PETG), a glycol-modified class of Polyethylene Terephthalate (PET), as a potential material for the fabrication of bone scaffolds. PETG scaffolds with a 0/90 lay-dawn pattern and different pore sizes (300, 350 and 450 µm) were produced using a filament-based extrusion additive manufacturing system and mechanically and biologically characterized. The performance of PETG scaffolds with 300 µm of pore size was compared with polycaprolactone (PCL). Results show that PETG scaffolds present significantly higher mechanical properties than PCL scaffolds, providing a biomechanical environment that promotes high cell attachment and proliferation.

Highlights

Biological analysis was carried out using Human adipose-derived stem cells, MesenPRO RSTM basal media, 2% (v/v) growth supplement, 1% (v/v) glutamine and 1% (v/v) penicillin/streptomycin, purchased from STEMPRO Invitroge, Thermo Fisher (Glasgow, UK) 2.2
Polyethylene Terephthalate (PET) is a common polymer used in many applications due to its high melting point, but it presents low printability
terephthalic acid (TPA) + CHDM peaks, the MALDI spectrum contains other peaks that correspond to fragment ions

Summary

Introduction

The scaffold-based approach, the most common strategy for bone tissue engineering applications, is based on the use of three-dimensional (3D) biocompatible and biodegradable porous structures that provide the substrate and the biomechanical environment for cells to attach, differentiate and proliferate [7,8,9,10,11,12]. A variety of biodegradable materials have been used to produce the scaffolds, including a wide range of organic, inorganic and composite materials [15,19,20,21]. Among these materials, polycaprolactone (PCL), an aliphatic polyester, was extensively used by our group for bone tissue engineering applications

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