The Potential of Orange Peel Oil as a Suppressor of Cell Proliferation in Animal Feed and Human Nutrition: An Experimental Study

Abstract

This study aimed to investigate the in vitro cytotoxic activities of orange peel oil on HaCaT cell lines by using an MTT cytotoxicity assay after administering orange peel oil at different doses and time-points. Our objective was to assess the in vitro cytotoxic activities of orange peel oil on HaCaT cell lines.‎ Cell viability was determined with the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] assays. The HaCaT cells (100 µL) were cultured in plates and treated with different concentrations of orange peel oil (25 µM, 50 µM, 100 µM, 150 µM and 200 µM) for durations of 24 and 48 hours. Cell death was determined by collecting and staining with 0.4% Trypan blue for 5 minutes at room temperature, followed by microscopic examination. There was a significant difference between the doses concerning ‎both time zones (p <0.05). There was a significant (p<0.05) difference between the control group and all other doses, including 200 µl/mL and 25 µl/mL, 50 µl/mL, 100 ‎µl/mL, and 20% DMSO. Orange peel oil showed toxic effects at all dose levels and time points when compared with the control group. According to the results of our research, and in light of previous investigations, it can be said that orange shell oil may have protective effects such as anti-cancer, anti-microbial, and antioxidant properties, and thus, may be used in human and animal nutrition.