Abstract
Routine medical microbiology diagnostics relies on conventional cultivation followed by phenotypic techniques for identification of pathogenic bacteria and fungi. This is not only due to tradition and economy but also because it provides pure culture needed for antibiotic susceptibility testing. This review focuses on the potential of High Resolution Melting Analysis (HRMA) of double-stranded DNA for future routine medical microbiology. Search of MEDLINE database for publications showing the advantages of HRMA in routine medical microbiology for identification, strain typing and further characterization of pathogenic bacteria and fungi in particular. The results show increasing numbers of newly-developed and more tailor-made assays in this field. For microbiologists unfamiliar with technical aspects of HRMA, we also provide insight into the technique from the perspective of microbial characterization. We can anticipate that the routine availability of HRMA in medical microbiology laboratories will provide a strong stimulus to this field. This is already envisioned by the growing number of medical microbiology applications published recently. The speed, power, convenience and cost effectiveness of this technology virtually predestine that it will advance genetic characterization of microbes and streamline, facilitate and enrich diagnostics in routine medical microbiology without interfering with the proven advantages of conventional cultivation.
Highlights
While molecular-genetic techniques have been rapidly implemented in detection and identification of microorganisms that cannot be cultivated, grow very slowly in culture or require special techniques, their use in the case of other microbes is still limited
For bacterial species that have no special requirements for growth, conventional cultivation followed by phenotypic identification still represents the golden standard in routine medical microbiology because of tradition and economy and because it provides pure culture needed for antibiotic susceptibility testing
We expect the routine availability of the newly available elegant technique of high-resolution melting analysis (HRMA) in medical microbiology laboratories should provide a strong stimulus in this field
Summary
Routine medical microbiology diagnostics relies on conventional cultivation followed by phenotypic techniques for identification of pathogenic bacteria and fungi. This is due to tradition and economy and because it provides pure culture needed for antibiotic susceptibility testing. This review focuses on the potential of High Resolution Melting Analysis (HRMA) of double-stranded DNA for future routine medical microbiology
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