Abstract
This work shows the potential of a very compact, portable Laser Induced-Breakdown Spectroscopy (LIBS) system that includes low cost and compact Nd:YAG laser, intended for use in fieldwork identification of bacteria. This device allows for the generation of 2 - 4 pulses per laser shot, with a maximum energy of 120 mJ per pulse. Each pulse has a duration of 60 ns, and the total duration of the train of pulses is 180 μs. The device dimensions including the power supply are 50 × 20 × 140 cm, which indicates that can be utilized as a portable LIBS system in field conditions. The spectra captured on two different bacteria shows the capability of identification.
Highlights
Identification of microbiological objects such as bacteria, fungi and pollens has become a critical task for medical, biological and environmental surveillance
We present the design and configuration of a very compact and economic multi-pulse laser that is completely immune to the alignment problem
We have developed a Nd:YAG laser with Q-switches and a saturable absorber for application in portable Laser Induced-Breakdown Spectroscopy (LIBS) for in-situ analysis
Summary
Identification of microbiological objects such as bacteria, fungi and pollens has become a critical task for medical, biological and environmental surveillance. Unlike other atomic emission spectroscopy methods, the source of excitation is a laser pulse, providing various advantages over other techniques Among these advantages, the system can detect almost any element, does not require prior sample preparation, and enables fast detection; in addition, because of its portability, it is possible to perform an in-situ or nearby analysis for inaccessible samples [4]. Q-switches are less expensive, more compact and have a longer lifespan than the cavity switching systems used to generate shorter pulses in double-pulse set-ups These works have proved that using more than one laser pulse to generate the plasma improves the signal intensity of the LIBS spectrum, which, in turn, improves the detection limit. Rapid identification of bacteria using a portable and inexpensive technique, would have great importance as it could effectively solve the existing demand for express determination in this field
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