Abstract
Potato Dextrose Agar (PDA) is the most media to grow the mushrooms, but the price of this media is expensive. Thus we need alternative media that easy and cheap to get it. This study aims to determine the growth of Aspergillus niger in alternative Artocarpus communis and Artocarpus heterophyllus seeds media. This research was an experimental study using a completely randomized design (CRD) one factor was the type of media is PDA (M0), Artocarpus communis seed media (M1), Artocarpus heterophyllus seed media (M2) and using the test mushrooms A. niger (J1). Inoculation of A. niger used agar block method for 3 days with a temperature of 28°C. Parameter of research was colony diameter and sporulation of A. niger. Data obtained with qualitative and quantitative methods. The result of this research showed that the best growth for Aspergillus niger was after 72 hours incubation. Colony diameter continually in PDA media, Artocarpus communis media, and Artocarpus heterophyllus media is 4.7 cm, 4.3 cm, and 4.1 cm with heavy sporulation. Therefore, Artocarpus communis and Artocarpus heterophyllus seeds media can be utilized as a substitution of PDA media for the growth of mushrooms.
Highlights
Medium is a material consisting of food substance mixture which functions as place for microbes to grow
A. niger growth on breadfruit seed and jackfruit seed as alternative medium show optimal result and the growth almost same with control medium (PDA). This is because breadfruit seed and jackfruit seed have the nutrients that needed for mushroom growth, especially carbohydrate
Based on result, derived mediums from breadfruit seed and jackfruit seed can be used as substitution or alternative medium of PDA (Potato Dextrose Agar) medium because those media can support mushroom growth
Summary
Medium is a material consisting of food substance mixture (nutrient) which functions as place for microbes to grow. Semi-synthetic media like PDA has sufficient carbohydrate so it is good for mushroom growth. This medium is needed a lot for mushrooms propagation in laboratory and agriculture. Carbon source from carbohydrate and protein are widely used by researchers to make alternative mushroom growth medium like cassava starch (Kwoseh et al, 2012); White and purple sweet potatoes, cocoyam and yam (Amadi et al, 2012); Cowpea, black soybeans and green beans (Ravimannan et al, 2014). There are many sources of carbohydrates from raw material and waste material those are cheap and easy to get but not yet optimized as mushroom culture medium, especially breadfruit seed and jackfruit seed. Based on the description above, researcher intends to utilize carbohydrate source from breadfruit seed and jackfruit seed as substitution of mushrooms growth medium. Data were analyzed with qualitative and quantitative methods by making observation mushrooms diameter of colonies and sporulation
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