Abstract
In this study, the Pluronic F127 with modified tripeptide Gly-Arg-Gly-Asp copolymer (hereafter defined as 3BE) hydrogel was evaluated in terms of its biocompatibility potentials. The fibroblasts (Swiss 3T3 cell line) and human hair follicles-derived mesenchymal stem cells (HFMSCs) were cultured in different concentrations of the 3BE hydrogel (0%, 0.05%, 0.1%, 0.25%, and 0.5%, respectively). The cell morphology and differentiation potential of HFMSCs were observed through optical microscopy, and the cell viability was investigated via Live/Dead Kit and Cell Counting Kit-8 assay. Analytical results showed that HFMSC can differentiate into adipogenic, chondrogenic, and osteogenic lineages. The HFMSC and Swiss 3T3 cells would properly assemble into a spherical shape as cultured with the 3BE hydrogel. Most importantly, cell viability could be maintained above 70%. The formation of spheroid structures of cells within this hydrogel is predicted to promote cell differentiation potentials of HFMSC that benefit in generating functional adipocytes, chondrocytes, and osteoblasts. Therefore, these findings demonstrate that the 3BE hydrogel has great potential as a three-dimensional cell culture scaffold for tissue engineering applications.
Highlights
Nowadays, massive advances have been made in research fields to improve cell culture techniques [1,2,3]
The results demonstrated that the isolated hair follicles-derived mesenchymal stem cells (HFMSCs) have the ability to differentiate into adipocytes, chondrocytes, and osteocytes
We evaluated whether the extracted mesenchymal stem cells (MSCs) from human hair follicles have multiple differentiation potentials
Summary
Massive advances have been made in research fields to improve cell culture techniques [1,2,3]. In a 2D cell culture, the microenvironment is not physiologically uniform due to the absence of nutrients, oxygen, and extracellular matrix (ECM), which leads to a failure in exhibiting the cell development process [6,7] Other limitations of this method, such as loss of cell mechanical and biochemical signals, loss of tissue-specific architecture, loss of cell-to-cell interactions, and loss of cell-to-matrix interaction have been reported [4,8,9]. Due to this fact, it is crucial to develop a suitable environment to properly nourish cells and preserve cell growth and proliferation [4,10]. Numerous modifications have been proposed to improve cell growth in three dimensions, some of them are increasing scaffold bioactivity and using a scaffold for cells [11,12]
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