Abstract
The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.
Highlights
Skin injuries are the most commonly found injury caused by blisters, burns, cuts, and ulcers of various severities [1]
A similar result was seen from the quantitative evaluation (Figure 2) when the HaCaT keratinocyte scratch assays were performed in mesenchymal stem cell-conditioned medium (MSC-conditioned medium (CM)), where there was a significantly enhanced rate of cell migration compared to the control
At the 72 h time point of the experiments, when the cell migration rate of the HaCaT keratinocyte scratch assays was 70% in the presence of Mesenchymal stem cells (MSCs)-CM, it was only 50% in the control medium. These findings indicate that MSC-CM enhanced the rate of wound closure of the
Summary
Skin injuries are the most commonly found injury caused by blisters, burns, cuts, and ulcers of various severities [1]. Any skin injury can interfere with its protective function and cause body tissues to be exposed to mechanical damages and microbial infections [2]. Other problems that may accompany wound healing are excessive healing, delayed wound healing, or lack of skin appendages [1]. The increasing demand for quality improvement of damaged skin has led to the shifting focus of wound repair to the restoration of anatomy and function of the skin [3]. The main purpose of wound care is to obtain wound closure at the earliest time [1] Identifying an effective approach to enhance the wound healing process is indispensable [1,2,3,4].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
