THE POTENTIAL FOR EVOLUTION OF SALT (NaCl) TOLERANCE IN SEVEN GRASS SPECIES

Abstract

SUMMARYThe seedling root growth of Agrostis stolonifera L., Agrostis capillaris L., Holcus lanatus L., Lolium perenne L., Dactylis glomerata L., Festuca rubra L. and Puccinellia distorts (L.) Parl. was examined after 14 d of immersion in dilute nutrient solutions containing 0, 150, and 250 mol m−3 NaCl. Root growth of all species was significantly reduced by increasing NaCl concentration.Samples of 10000 to 20000 seeds of each species were then screened for ability to root in solution cultures at NaCl concentrations which markedly inhibited root growth in the first experiment. Small numbers of individuals of all species rooted in these solutions and high and low selection lines were established based upon root length values.Adult plants from these high and low selected lines were compared with an unselected sample for ability to root in a range of NaCl concentrations. The high line plants had significantly longer roots than the unselected plants in all species, and than the low selected line in all species except Agrostis capillaris. Low selected plants had significantly longer roots than unselected plants in Holcus lanatus, and Dactylis glomerata.The pattern of response to selection showed no consistent relationship to the known ecology of the species examined, and suggests that variability in salt tolerance is widely present in species, but for other reasons salt tolerance does not always evolve in natural situations.