Abstract
Background and Aim: To investigate whether and how inhibition of GSK-3 regulates myocardin activity in the human vascular smooth muscle cells (SMCs), and to explore the action of GSK-3 inhibitor (AR-A014418) on atherosclerosis (AS) in rats. Materials and Methods: The western-blotting and RT-qPCR were conducted to gauging the changes in protein and transcription levels of related factors. Immunoprecipitation was used to discuss the relationship between myocardin and ACTA2. HE staining and red oil staining were performed to demonstrate pathological changes in renal tissue and aorta. Results: AR-A014418 sharply reduced the activity of myocardin and further abated its phosphorylation of serine and threonine and the recruitment of myocardin to ACTA2. Besides, AR-A014418 could alleviate the pathological changes and significantly increase expression levels of ACTA2 in AS rats. Conclusion: GSK-3 may bilaterally regulate the transcriptional activity of myocardin in vascular SMCs. The regulation of GSK-3 on the transcriptional activity of myocardin may be an important mechanism of differentiation and dedifferentiation of vascular smooth muscle and related diseases.
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