Abstract
We examined the susceptibility of &delta;<sup>15</sup>N-signals and C<sub>org</sub>/N ratios in organic-rich sediments to pre-analysis sample treatment. Each sample was subjected to three different kinds of processing. For comparative purposes, the first measurement series (MS-1) was carried out on untreated sediment. In MS-2, the sediment was rinsed with distilled water. In MS-3, analyses were carried out on decalcified and rinsed material, in MS-4 the samples were decalcified without being subsequently washed. The sediment yielded conspicuously different results depending on the type of processing it was subjected to. Rinsing, irrespective of whether acidification was included or not, induced substantial modifications in &delta;<sup>15</sup>N accompanied by a pronounced loss of NH<sub>4</sub><sup>+</sup> (up to 14 wt% of the initial N-content). Molar C<sub>org</sub>/N ratios, on the other hand, were only affected by a combination of acidification and rinsing. The discrepancies are ascribed to the influence of decomposition-derived ammonium (NH<sub>4</sub><sup>+</sup>). In untreated sediment (MS-1), NH<sub>4</sub><sup>+</sup> seems to produce misleading shifts in both &delta;<sup>15</sup>N-signals and C<sub>org</sub>/N ratios. Presumed mechanisms involved are as follows: Firstly, nitrogen isotopes fractionate during NH<sub>4</sub><sup>+</sup>-volatilisation in the heating oven, where the sediment is put to desiccate. Secondly, NH<sub>4</sub><sup>+</sup>-ions are able to escape that fractionation when adsorbed to negatively-charged SiO<sub>2</sub>-surfaces. The adsorption capacity of SiO<sub>2</sub> increases with increasing pH of the pore water and hence with increasing carbonate content. Our findings raise serious doubts about whether untreated sediment (MS-1) can provide reliable C<sub>org</sub>/N and &delta;<sup>15</sup>N-records. Pre-analysis acidification plus rinsing (MS-3) seems to eliminate the deceptive influence of NH<sub>4</sub><sup>+</sup>-adsorption and -outgassing. <br><br> doi:<a href="http://dx.doi.org/10.1002/mmng.201100004" target="_blank">10.1002/mmng.201100004</a>
Highlights
Nitrogen isotope abundance ratios (d15N) and C/N ratios are both important instruments in the study of marine food webs
The goal of this study is to investigate the effects of common processing methods on the organic-rich sediments from the central Namibian shelf and to establish which can be most trusted with respect to the d15N- and C/N record
A study of the available literature shows that the effects of sample preparation on d15N and C/N are far from unanimous
Summary
Nitrogen isotope abundance ratios (d15N) and C/N ratios are both important instruments in the study of marine food webs. Their value – as with every proxy indicator – depends on the reliable recording of the original phenomenon in the absence of the direct measure. In this regard, the deceptive influence of sample preparation on the original C/N ratios and d15N-signals is of great interest and has already been addressed by several authors (Nieuwenhuize et al 1994; Bunn et al 1995; Holmes et al 1999; Jacob et al 2005; Kennedy et al 2005; Carabel et al 2006; Ng et al 2007). The goal of this study is to investigate the effects of common processing methods on the organic-rich sediments from the central Namibian shelf and to establish which can be most trusted with respect to the d15N- and C/N record
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