Abstract
Stahlianthus involucratus (S. involucratus) has anti-inflammatory, antinociceptive, and antipyretic activities; however, there are no literature reports on its antioxidant capacity. This study presents a comparative assessment of the polyphenols contents, flavonoids contents, and antioxidant activity of the aqueous and methanol extracts of S. involucratus (ASI and MSI). Moreover, the expression of oxidative stress-related genes in H2O2-induced H9c2 cells pretreated with the MSI was measured by RT-qPCR, and furthermore, MSI were characterized by UHPLC-Q-Orbitrap-MS/MS. The results indicated that the MSI had higher antioxidant contents and antioxidant capacity, and MSI could inhibit H2O2-induced oxidative stress in H9c2 cells by activating the Nrf2/HO-1 pathway. UHPLC-Q-Orbitrap-MS/MS characterized 15 phenolic compounds from the MSI. In conclusion, S. involucratus has the potential antioxidant capacity.
Highlights
S. involucratus, belonging to Zingiberaceae [1], as a Traditional Chinese Medicine (TCM), was mainly used for the treatment of bruises and rheumatic arthralgia [2]
This study presents a comparative assessment of the polyphenols contents, flavonoids contents, and antioxidant activity of ASI and MSI, and the expression of Nqo1, Ho1, Gclc, Gclm, Gst, and Nfe212 in H2O2-induced H9c2 cells pretreated with the MSI was examined by RT-qPCR, and the phytochemical compounds from MSI were isolated and characterized by Ultrahigh performance liquid chromatography (UHPLC)-Q-Orbitrap-MS/MS
MSI were analyzed by UHPLC-MS/MS spectrum consisting of Thermo ScientificTM, UltimateTM 3000RS, Thermo ScientificTM, Q ExactiveTM, Hbrid Quadrupole-Orbitrap mass spectrometer, ESI source, and RP-C18 column (150 × 2:1 mm, 1.8 μm)
Summary
S. involucratus, belonging to Zingiberaceae [1], as a Traditional Chinese Medicine (TCM), was mainly used for the treatment of bruises and rheumatic arthralgia [2]. The reporter verified that cardamonin from the Alpinia plant inhibits oxidative stress, apoptosis, and inflammatory responses in the mouse heart by improving NRF2 signaling [8]. Excessive secretion of free radicals causes oxidative damage to cells and tissues, induces oxidative stress, and further leads to inflammatory responses and damages the immune system [9]. The activated NRF2 binds to antioxidant response elements (ARE) to regulate downstream genes and defend against damage caused by oxidative stress. A negative effect of free radicals in the body, is associated with various diseases, including inflammatory response, cell damage, tissue sclerosis, and cancer [11]. This study presents a comparative assessment of the polyphenols contents, flavonoids contents, and antioxidant activity of ASI and MSI, and the expression of Nqo, Ho1, Gclc, Gclm, Gst, and Nfe212 in H2O2-induced H9c2 cells pretreated with the MSI was examined by RT-qPCR, and the phytochemical compounds from MSI were isolated and characterized by UHPLC-Q-Orbitrap-MS/MS
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