THE POTENCY OF JAVANESE TURMERIC (CURCUMA XANTHORRHIZA ROXB.) ETHANOL EXTRACT TO ERADICATE WILD STRAIN CANDIDA ALBICANS BIOFILM

Ayu Diah Lestari,Ria Puspitawati,Ariadna Adisattya Djais

doi:10.22159/ijap.2019.v11s1.150

Ayu Diah Lestari, Ria Puspitawati + Show 1 more

Open Access

https://doi.org/10.22159/ijap.2019.v11s1.150

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Abstract

Objective: The pathogenic yeast Candida albicans forms biofilm to increase its resistance toward antifungal agents. Javanese turmeric is an Indonesianmedicinal plant reported to have antifungal effects due to the active component, xanthorrhizol. The objective of this study was to measure the in vitropotential of Javanese turmeric ethanol extract to eradicate C. albicans biofilm.Methods: C. albicans was exposed to Javanese turmeric ethanol extract for 1 h during biofilm formation phases. MTT assay was used to test thepercentage of biofilm eradication.Results: The minimum inhibitory concentration and minimum fungicidal concentration of Javanese turmeric ethanol extract against planktonicC. albicans were 15%. The minimum biofilm eradication concentration (MBEC50) was 25% in the early phase and 15% in the intermediate andmaturation phases.Conclusions: Javanese turmeric ethanol extract is effective at eradicating clinical isolate of C. albicans biofilm.

Highlights

Oral candidiasis is an opportunistic infection caused by Candida albicans fungal infection
Samples of C. albicans were obtained from individuals who met the criteria exposed to 100,000 IU nystatin as a positive control and from individuals who met the criteria not exposed to Javanese turmeric ethanol extract or nystatin as a negative control
The results showed germ tube formation in clinical isolates after incubation at 37°C for 2 h (Figs. 1 and 2)

Summary

Introduction

Oral candidiasis is an opportunistic infection caused by Candida albicans fungal infection. Changes in C. albicans characteristics can be caused by predisposing factors in the host, such as use of broad-spectrum antibiotics or corticosteroids, systemic conditions such as HIV/AIDS and diabetes mellitus, malnutrition, removable dental appliances, and decreases in saliva flow [1]. Some virulence factors of C. albicans can increase its pathogenic characteristics. One of these is the ability to adhere and form biofilms in host tissue. The biofilm structures consist of yeast, germ tubes, and young hypha with an extracellular matrix. The extracellular matrix expands; the incubation time increases; and increasing yeast, pseudohypha, and hypha are planted in the matrix [2]

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