The potency of flavonoid n-hexane, chloroform, and ethanolic fraction from Scurrula atropurpurea (Blume) danser on proliferation and apoptosis through methylation of p16 gene on HeLa cells

Abstract

Human Papilloma Virus (HPV) infection suggested could induced gene hypermethylation. Hypermethylation occurs on the p16 tumor suppressor gene promoter resulted in silencing tumor suppressor gene so that the gene is inactive and contributes to the malignancy of cancer cells. Tea parasites or Loranthaceae are known to be a plant that has an anticancer potency, correlated to flavonoids content. Flavonoid from Scurrula atropurpurea (Blume) Danser (SAD) suggested have a role in inhibiting methylation towards p16. This research objective was to identify the potency of Lawang - East Java SAD flavonoids compound towards Hela cells. In this study, we examine flavonoids from SAD that been extracted in n-hexane, chloroform, ethanol solvent, using TLC Chromatogram, and LCMS. We also examined the cells proliferation using MTT assay, the apoptotic level was examined by Tunnel, and then completed with methylation level detection using bisulfite direct sequencing method. The result showed that flavonoids that found were: flavanone, dihydro flavonol, and flavon, dihydro flavonol, flavanone and catechin, and flavonol, flavon, and EGCG, respectively n-hexane, chloroform and ethanol fraction. Flavonoids have proliferation inhibitor and apoptotic inductor potency via inhibition of p16 promoter of HeLa cells methylation. SAD flavonoids in the n-hexane and chloroform fraction shown the most potent in methylation inhibition.