Abstract
Flavonoids can provide antioxidant effects by preventing the formation of ROS, directly capture ROS, protect lipophilic antioxidants and stimulate the increase of enzymatic antioxidants. Flavonoids are phenolic compounds that are widely found in medicinal plants, one of which is Gyrinops versteegii leaves. In this research to determine the potential of flavonoids in water extract Gyrinop versteegii as one source of natural antioxidants was investigated. This research begins with maceration of Gyrinops versteegii leaves with some solvents such as ethyl acetate, ethanol, methanol and water. Each of the extracts obtained measured the total content of Phenol. The extract, which had the highest total phenol content, measured the total flavonoid content and antioxidant capacity. The active extract as antioxidant was further isolated and identified its flavonoid content. Flavonoids obtained measured antioxidant capacity in vitro. Total phenol (mg GAE/100 g) of ethyl acetate extract = 443, ethanol extract =1.510, methanol extract = 6.069 and water extract = 14.979, total flavonoid contens = 2298, 977 mg QE/100 gram, containing phenol, flavonoid, tannin, alkaloid and steroid compounds. Antioxidant capacity with IC50 = 3,45 ppm (5 min.) and 3,05 (60 min.). Identification of isolates with UV-Vis spectroscopy showed 2 absorption bands namely band I at 352 nm and band II at 256 nm. Addition of AlCl3 / HCl shear reagent showed band I undergoing a 2 nm batochromic shift. These results indicate that the resulting flavonoid is suspected to be a flavonoid group of flavonol substituted -OH group at C-5 or 5-hydroxy-flavonol. and its antioxidant capacity or IC50 = 17,14 ppm. These results indicate that the isolated flavonoid has very strong antioxidant activity and is potentially developed as a natural antioxidant.
Highlights
The utilization of medicinal plants or herbs in tackling health problems is increasing
The body is already producing or endogenous antioxidant such as superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) but has not been able to deal with the excess of free radicals in the body resulting in an imbalance of antioxidant production and the amount of free radicals (Akhlaghi, et al, 2009)
In addition to vitamins contained in vegetables and fruits some phenolic compounds such as flavonoids that of the medicinal plants can be used as a natural antioxidant
Summary
Material Fresh Gyrinops versteegii leaves, obtained from the village of Marga, Subdistrict Marga, Tabanan, Bali, Indonesia, male rats Wistar from BBVet , standard food of, ethanol GR(E Merck), ethyl acetate GR (E Merck), HCl GR (E Merck), NHCO3 GR(E Merck), NH4Cl GR (E Merck), methanol GR (E Merck), TCA, TBA, TEP, BHT (Sigma), PBS, xanthin oxidase, Na-EDTA, H2O2, BSA 0,5%, 2.5 mM NBT, MDA Assay Kit from NWK Northwest, CMC-Na and Whatmann Filter Paper No.[4] (E Merck) Equipment. UV-Vis spectrophotometer (Varian), analytic Digital Balance Scale (Ohaus), Brand Memmert oven, polypropilin tube (Colom 18), rotary vacuum evaporator Brand Buchii, Vortex, water bath
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