The postnatal ontogeny of substance P-immunoreactive fibers in the sympathetic preganglionic nuclei of the rat

Abstract

The ontogeny of substance P (SP)-containing fibers and puncta is described in laminae VII and X of the rat thoracolumbar spinal cord from the day of birth until postnatal day (P) 60. As SP fibers and puncta innervate and demarcate the distribution of preganglionic sympathetic nuclei, strong temporal and weak rostrocaudal ontogenetic gradients exist. Additionally, a heterogeneous segmental SP ontogenetic pattern is observed in sympathetic nuclei. On the day of birth, SP fibers are present in an unorganized fashion in sympathetic nuclei with the exception of the nucleus intercalatus which is clearly outlined. From P0 to P4 SP fibers and puncta are established along a ‘ladder-like’ pattern and from P6 to P15 SP fibers and puncta steadily accumulate in sympathetic nuclei at all spinal levels. By P15 the nuclei intermediolateralis, pars principalis and funicularis, and the nucleus intercalatus are clearly outlined by SP immunoreactivity while the central autonomic region (lamina X) contains heterogeneous bands of SP immunoreactivity. From P20 to P30, SP fibers and puncta accumulate in each autonomic nucleus and longitudinal SP connections form between each adjacent nucleus intermediolateralis pars principalis. Along the spinal midline the nucleus intercalatus pars paraependymalis and the dorsal commissural nucleus emerge from the central autonomic region as separate SP-innervated nuclei. On P40 a period of reorganization takes place so that SP fibers within sympathetic nuclei become more diffuse and the nucleus intercalatus becomes more complex in appearance. The adult SP pattern is formed by P60 when the dorsal commissural nucleus dense core becomes the last autonomic region to be SP innervated. In addition, a transient population of immunohistochemically demonstrable cervical-thoracic laminae VII and X SP cell bodies are observed from P2 to P15.