Abstract
Summary 1. The rate of oxidation of glucose C14 to C14O2 in a standard incubation system is proposed as an index of tissue viability. The mean of 74 observations on fresh dog liver is 2.89 ± 0.45 μM. of glucose oxidized to CO2 per gram of wet liver per 100 minutes, as based on recovery of radiocarbon in carbon dioxide. 2. Liver tissue that has been stagnant at room temperature for 24 hours has activity approximately 12 per cent of normal. 3. Use of an oxygen-free atmosphere of nitrogen reduces the activity to 1 per cent of the mean. 4. The corrected (100 minute) activity rate for carbon dioxide production is the same when the incubation is carried on for periods of 85 to 230 minutes. 5. This incubation procedure does not alter significantly the glycogen content of the liver slices, nor their histologic architecture. 6. Some of the advantages and potential dis-advantages of the use of this index of tissue viability are discussed.
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