The Possible Protective Role of N-Acetyl Cysteine against Titanium Dioxide Nanoparticles Intestinal Toxicity in Adult Male Albino Rats

Eman Khayal,Marwa Shabana,Dalia Amin

doi:10.21608/ajfm.2019.36575

Eman Khayal, Marwa Shabana + Show 1 more

Open Access

https://doi.org/10.21608/ajfm.2019.36575

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Abstract

Introduction: With extensive applications of nanoparticles including titanium dioxide nanoparticles (TiO2 NPs) in different fields, many adverse effects may threat both environmental and medical health. Aim of this work: To evaluate the role of n-acetyl cysteine (NAC) against TiO2 NPs intestinal toxicity in adult albino rats. Material and methods: Fifty adult male albino rats were classified into five groups. Group I: negative control received regular diet and water. Group II: positive control administered 1ml of 5% gum acacia solution once a day. Group III: gavaged orally with NAC (100 mg/kg). Group IV: orally gavaged with TiO2 NPs (1200 mg/kg) once a day. Group V: orally gavaged once daily with TiO2 NPs (1200 mg/kg) and NAC (100 mg/kg). After 6 weeks, all rats were weighed and blood sample were submitted to estimate serum level of citrulline. Then parts of ileum from all rats was dissected and subjected to interleukin 6 (IL6), interleukin 10 (IL10) and tumor necrosis factor (TNF-α) estimation, histopathological examination and determine the extent of DNA damage by gel electrophoresis. Results: The results revealed that TiO2 NPs induced significant decreases in body weight, and serum level of citrulline with significant increases in IL6, TNF-α and significant decrease IL10 in tissues of ileum. These were associated with histopathological changes in the ileum and DNA damage detected by gel electrophoresis. Co-administration of NAC with TiO2 NPs provided protection against these changes. Conclusion: TiO2 NPs exposure causes inflammation in ileum with DNA damage and administration of NAC offers protection against its damaging effects.

Highlights

With extensive applications of nanoparticles including titanium dioxide nanoparticles (TiO2 NPs) in different fields, many adverse effects may threat both environmental and medical health
The results revealed that TiO2 NPs induced significant decreases in body weight, and serum level of citrulline with significant increases in interleukin 6 (IL6), TNF-α and significant decrease interleukin 10 (IL10) in tissues of ileum
Addition of n-acetyl cysteine (NAC) to TiO2 NPs provided significant protection against body weight loss caused by TiO2 NPs (P

Summary

Introduction

With extensive applications of nanoparticles including titanium dioxide nanoparticles (TiO2 NPs) in different fields, many adverse effects may threat both environmental and medical health. Group V: orally gavaged once daily with TiO2 NPs (1200 mg/kg) and NAC (100 mg/kg). TiO2 NPs interact with cytoplasmic proteome nanoparticles with the environment inducing adverse producing posttranslational modifications, like effects on both environmental and medical health acetylation and oxidative stress (Sund et al, 2014). Inside the nucleus, have smaller size, large number of atoms on their they react with DNA (Park et al, 2008) and cause the surface and different surface characteristics, as level of upregulation of cytokines, oxidative stress, and energy. These differences attract more attentions to apoptosis (Gui et al, 2011)

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