The possible ameliorative effects of vitamin E against cisplatin-induced injury on adult rat liver and testes

Abstract

Cisplatin is one of the most potent cytotoxic drugs used to treat cancer, but clinical use is linked to testicular and liver damage. According to a number of studies, antioxidant supplementation may have an impact on the toxicity caused by cisplatin. The purpose of the current investigation was to determine how vitamin-E supplementation protected rats from cisplatin-induced damage. Thirty laboratory adult male albino rats were divided into three groups: Group I received saline orally, once daily for 21 days. Group II received cisplatin on day 0, 7, 14 and were sacrificed on day 21. Group III received cisplatin on day 0, 7, 14 and received orally vitamin E daily, starting 5 days before the first dose of cisplatin until day 21. Liver and both testes were obtained and fixed. Sections from the liver and both testes were stained by H&E and Trichrome stain, and then examined under light microscope. Alterations included a significant increase in malondialdehyde (MDA) level in the cisplatin group compared with the other groups (p value for comparing between control and each other group, statistically significant at p ≤ 0.05). Histopathologically, cisplatin induced signs of hepatic injury; it also showed signs of testicular degeneration in all rats. However, the cisplatin induced disturbances significantly improved by treatment with Vitamin E. Statistical analysis showed a significant difference among the three groups in all signs of injury (p<0.001). According to this research, cisplatin has a toxic impact on the liver and testicles, and when it is administered along with vitamin E, a noticeable improvement is seen.