Abstract
Production of flavonoids in madder callus culture (Rubia tinctorum L.) was dependent on culture conditions and culture media composition. The content of flavonoids increased in calli maintained on media supplemented with NAA (4 mg.l-1) or NAA:BAP (4 mg.l-1 a 1 mg.l-1) in 16 h photoperiod. Flavonoids represented 2.08 – 2.25 % of callus dry mass. The presence of Cd(NO3)2 (3.1 or 31.0 mg.l-1 concentrations) negatively influenced callus growth, but enhaced the percentage of dry mass in callus cells. During 42 days of culture an increase of cadmium accumulation and even of flavonoids has been observed. The most considerable influence of CdCl2 or Cd(NO3)2 on flavonoids content has been shown in short-term experiments after 48 h of callus culture. More distinct influence has been observed under the treatment with CdCl2 (0.005 mg.l-1) in comparison with Cd(NO3)2.
Highlights
The effort to use natural resources of vegetal and animal origin supports detailed investigation of familiar plant species for discovery of their still unknown impact against negative factors of the environment
Besides standard application and isolation of secondary compounds from natural resources, increasing significance win biotechnologies aimed on bioactive metabolites production and enhancement of their production abilities, predominantly plant cell cultures in vitro (PŠENÁKOVÁ et al, 2003; VANISREE and TSAY, 2004; VANISREE et al, 2004)
The aim of this study was to detect the potential of madder (Rubia tinctorum L.) callus cultures to produce flavonoids in dependency on culture media composition, physical conditions, combination and concentration of growth regulators, and the effect of Cd-salts - Cd(NO3)2 and CdCl2
Summary
The effort to use natural resources of vegetal and animal origin supports detailed investigation of familiar plant species for discovery of their still unknown impact against negative factors of the environment. Cultivation of medicinal plants is in the focus worldwide. This is evoked by the appearance of numerous civilization deseases, and by the new look on prophylaxis and food health. Prominent plant species contain secondary metabolites usable in food industry, cosmetics, and mainly in pharmaceutical industry. Besides standard application and isolation of secondary compounds from natural resources, increasing significance win biotechnologies aimed on bioactive metabolites production and enhancement of their production abilities, predominantly plant cell cultures in vitro (PŠENÁKOVÁ et al, 2003; VANISREE and TSAY, 2004; VANISREE et al, 2004). Cell and callus cultures represent a continual renewable source of plant biomass and unlimited resource of desired pharmaceutical products
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