The possibility of using bacteria Bacillus in the production of disinfectants

Abstract

Introduction. In recent years, healthcare-associated infections (HAIs) cause ever greater economic and social damage. Microorganisms circulating in medical institutions the causative agents of HAIs, become resistant to most antibiotics and many disinfectants. There is a need to create alternative mechanisms for controlling microorganisms. The purpose of the work is to determine the antagonistic activity of bacteria strains of the genus Bacillus in relation to test microorganisms, as a potential basis for the development of domestic detergents and disinfectants based on probiotics. Material and methods. The strains of Bacillus subtilis 2/10 VKPM B-2896; Bacillus subtilis VKPM B-1283; Bacillus licheniformis VKPM B-5397; Bacillus amyloliquefaciens VKPM B-5462 werethe main object of study. At the first stage of the studies, the antagonistic activity of the strains was studied by the well method, at the second stage of the studies, the antagonistic activity was studied at the test object, and at the third stage of the studies, the effectiveness of the composition containing B. subtilis VKPM B-1283, B.licheniformis VKPM B-5397 and sulfonol strains was studied. Results. As a result of studies, the antagonistic effect of bacilli against test microorganisms was revealed. When surfaces are contaminated with test microorganisms (S.aureus ATCC 6538-P, E.coli ATCC 10531, C. albicans ATCC 10231) and subsequent application of Bacillus spores, a significant reduction in the seeding rate with test microorganisms is observed after 24 hours by 97.07-100%. The use of B.subtilis VKPM B-1283 and B.licheniformis VKPM B-5397 spores together with a detergent component for treating surfaces contaminated with test microorganisms (S.aureus ATCC 6538-P, E.coli ATCC 10531, C.albicans ATCC 10231) provides a reduction in microbial seeding after 4 hours by 99.97 %. Conclusions. B.subtilis 2/10 VKPM B-2896, B.subtilis VKPM B-1283 and B.licheniformis VKPM B-5397 are promising for the use as probiotics, as the basis of detergents and disinfectants.