The positive function of selenium supplementation on reducing nitrate accumulation in hydroponic lettuce (Lactuca sativa L.)

Abstract

High nitrate (NO3−) in vegetables, especially in leaf vegetables poses threaten to human health. Selenium (Se) is an important element for maintaining human health, and exogenous Se application during vegetable and crop production is an effective way to prevent Se deficiency in human bodies. Exogenous Se shows positive function on plant growth and nutrition uptake under abiotic and/or biotic stresses. However, the influence of exogenous Se on NO3− accumulation in hydroponic vegetables is still not clear. In the present study, hydroponic lettuce plants were subjected to six different concentrations (0, 0.1, 0.5, 5, 10 and 50 µmol L−1) of Se as Na2SeO3. The effects of Se on NO3− content, plant growth, and photosynthetic capacity of lettuce (Lactuca sativa L.) were investigated. The results showed that exogenous Se positively decreased NO3− content and this effect was concentration-dependent. The lowest NO3− content was obtained under 0.5 µmol L−1 Se treatment. The application of Se enhanced photosynthetic capacity by increasing the photosynthesis rate (Pn), stomatal conductance (Cs) and the transpiration efficiency (Tr) of lettuce. The transportation and assimilation of NO3− and activities of nitrogen metabolism enzymes in lettuce were also analysed. The NO3− efflux in the lettuce roots was markedly increased, but the efflux of NO3− from the root to the shoot was decreased after treated with exogenous Se. Moreover, Se application stimulated NO3− assimilation by enhancing nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS) and glutamate synthase enzyme (GOGAT) activities. These results provide direct evidence that exogenous Se shows positive function on decreasing NO3− accumulation via regulating the transport and enhancing activities of nitrogen metabolism enzyme in lettuce. We suggested that 0.5 µmol L−1 Se can be used to reduce NO3− content and increase hydroponic lettuce yield.