The positive contribution of natural deep eutectic solvent constructed by trehalose and fructooligosaccharides on rheological and 3D printing properties of mirror carp surimi

Abstract

To increase the diversity and stability of surimi products, this study developed polysaccharide-based water-tailored natural deep eutectic solvent (NADES) for the development of surimi 3D printed products. As the mass ratio of trehalose to fructooligosaccharides increased (1:1, 5:4, 4:3, 3:2, 2:1, 3:1), the liquid state of NADES first became clear and then turned turbid. At a mass ratio of 3:2, NADES appeared pure and free of impurities under polarized light microscopy. It was confirmed by FTIR and NMR spectroscopy that trehalose and fructooligosaccharides interact through hydrogen bonding to form NADES. Adding water to NADES significantly reduced its viscosity (P < 0.05), although this also gradually weakened the hydrogen bonds. At a 14.52% water addition, NADES exhibited relatively low viscosity and high hydrogen bond strength. Increasing the amount of NADES (0, 2, 4, 6, 8%) led to a rise in the viscosity, storage modulus, loss modulus, and tanδ of the surimi, and a denser three-dimensional network structure was observed via SEM. Under optimized 3D printing parameters (nozzle diameter of 1.2 mm, layer height of 1.0 mm, fill percentage of 75%, and print speed of 30 mm/s), surimi containing 4% NADES demonstrated the finest textures, achieving the highest print accuracy (99.33%) and stability (99.31%) among all samples tested. This study provides a theoretical basis for developing polysaccharide-based NADES and enhancing surimi product quality.