Abstract
PoxO1, FoxO3a and FoxO4 belong to the FoxO gene family, which play important roles in the PI3K/PKB pathway. In this study, we cloned the porcine FoxOl, FoxO3a and FoxO4 sequences and assigned them to SSCllpll-15, SSC1p13 and SSC xq13 using somatic cell hybrid panel (SCHP) and radiation hybrid panel (IMpRH). RT-PCR results showed that these three genes are expressed in multiple tissues. Sequencing of PCR products from different breeds identified a synonymous T/C polymorphism in exon 2 of FoxO3a. This FoxO3a single nucleotide polymorphism (SNP) can be detected by AvaII restriction enzyme. The allele frequencies of this SNP were investigated in Dahuabai, Meishan, Tongcheng, Yushan, Large White, and Duroc pigs. Association of the genotypes with growth and carcass traits showed that different genotypes of FoxO3a were associated with carcass length and backfat thickness between 6th and 7th ribs (BTR) and drip loss (p<0.05).
Highlights
Forkhead-box O (FOXO) transcriptional factors are members of the large forkhead family protein
Since the Drosophila fkh gene was cloned in 1984, many FOX family genes have been identified in various species (Kaestner et al, 2000)
Forty-three human FOX family proteins are classified into 17 subfamilies (FOXA-FOXQ) (Masuko and Katoh, 2004)
Summary
Forkhead-box O (FOXO) transcriptional factors are members of the large forkhead (fkh) family protein. Forty-three human FOX family proteins are classified into 17 subfamilies (FOXA-FOXQ) (Masuko and Katoh, 2004) Among these families, the FOXO subgroup contains four members, FOXO1 (FKHR), FOXO3a (FKHRL1), FOXO4 (AFx) and FOXO6 (Tran et al, 2003; Masuko and Katoh, 2004). RACE was we cloned three porcine FoxO genes, mapped them to performed according to the instructions of the SMARTTM chromosomes, find a SNP in FoxO3a and detected its RACE cDNA Amplification Kit Isolation of the porcine FoxO1, FoxO3a and FoxO4 primer pair F3a-PL3 and F3a-PR3 to map this gene. Hybrid panel (IMpRH) consisting of 118 hybrid clones PCR primer pairs were F1-PL2 and F1-PR for FoxO1, F3a-.
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