Abstract
The major obstacle of blood-contacting medical devices is insufficient hemocompatibility, particularly thrombogenicity and platelet activation. Pre-clinical in-vitro testing allows for the evaluation of adverse thrombogenicity-related events, but is limited, among others, by the availability and quantity of human blood donations. The use of animal blood is an accepted alternative for several tests; however, animal and particularly abattoir blood might present species-specific differences to human blood as well as elevated blood values, and pre-activated platelets due to stressed animals and non-standardized blood collection. To this end, we investigated porcine abattoir blood in comparison to human donor blood with the focus on platelet pre-activation and remaining activation potential. By means of light transmission aggregometry, aggregation kinetics of platelet rich plasma after stimulation with three different concentrations of each adenosine diphosphate (ADP) (5µM, 10µM, 20µM) and collagen (2.5µg/ml, 5µg/ml, 10µg/ml) were monitored. The activation with collagen revealed no significant differences in platelet behavior of the two species. In contrast, stimulation with ADP resulted in a lower maximum aggregation and a high disaggregation for porcine abattoir blood. The latter is a species-specific phenomenon of porcine platelets. Variations within each study cohort were comparable for human and abattoir pig. The similarities in platelet activation following collagen stimulation and the preservation of the porcine-specific reaction to ADP prove a general functionality of the abattoir blood. This finding provides a first step towards the complete validation of the porcine abattoir blood model.
Highlights
A growing percentage of elderly people in combination with a rising life expectancy increases the need for better and advanced medical care
light transmission aggregometry (LTA) was performed with human donor and porcine abattoir blood
Different concentrations of the activators adenosine diphosphate (ADP) and collagen were applied, and intra-species comparisons of concentrations and inter-species differences were analyzed with the focus on the usability of abattoir blood for in-vitro platelet activation and thrombogenicity testing
Summary
A growing percentage of elderly people in combination with a rising life expectancy increases the need for better and advanced medical care. Stimulation with ADP resulted in a lower maximum aggregation and a high disaggregation for porcine abattoir blood The latter is a species-specific phenomenon of porcine platelets. Conclusion: The similarities in platelet activation following collagen stimulation and the preservation of the porcine-specific reaction to ADP prove a general functionality of the abattoir blood. This finding provides a first step towards the complete validation of the porcine abattoir blood model
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