The population structure of Beijing family of Mycobacterium tuberculosis in Western Siberia

Abstract

The objective: to study the structure of Beijing genotype family of Mycobacterium tuberculosis in Omsk Region, Western Siberia. Subjects and methods : 325 strains of M. tuberculosis were studied, all of them were isolated from tuberculosis patients in Omsk Region. Strains were tested to identify if they belonged to Beijing genotype, B 0/W 148 and 9 4-3 2. Locus NTF and region RD 181 were assessed. Ancient Beijing strains were analyzed by spoligotyping and MIR U - V NT R -t yped at 12 loci, and the obtained profiles were compared with the international database of SITVIT _ WEB . Results . Among 204 (62.7%) strains of M. tuberculosis from Beijing family, 176 (86.3%) of strains belonged to the modern sublineage of 9 4-3 2 (60.7%) and B 0/W 148 (25.0%) clusters. Inside 9 4-3 2 cluster, it was found that 13 (10.5%) strains belonged to CAO group. Strains of the ancient Beijing sublineages of RD 181[+] and RD 181[-] were detected in 3.4% ( 7/2 04) and 10.3% (2 1/2 04) cases, respectively. All of Beijing RD 181[+] strains had SIT 269 spoligoprofile. The majority of strains of the ancient sublineage were represented by the MIRU types – MIT 135 and MIT 642. Conclusion. In Omsk Region of Western Siberia, strains of the modern sublineage prevail in the population of M. tuberculosis of Beijing family, the prevalence of ancient strains of this family is (13.7%). The high level of multiple drug resistance is typical of strains of the modern sublineage of B 0/W 148, 9 4-3 2 CAO clusters and strains of the ancient Beijing sublineage.