The pontine substrate of circling behavior

Abstract

Single or twin moveable stimulating electrodes were implanted in 9 rats in order to investigate the pontine substrate of circling behavior. The region located between the caudal part of the interpenduncular nucleus and the fourth ventricle was examined. The electrodes were implanted 6 mm below the surface of the skull and subsequently moved down by steps of 0.13 or 0.16 mm. The stimulating current consisted of trains of cathodal rectangular pulses of constant intensity and width (100 μA and 0.1 ms respectively) and of variable frequency. The effectiveness of the stimulation in eliciting a circling reaction was inferred from a psychophysical determination of the pulse period required at each site in order for the animal to maintain a criterion rotation speed. In the average, 48 brain sites were investigated per animal. Stimulation of 166 out of a total of 387 sites elicited ipsiversive rotation. Depending on the coronal plane of implantation, the dorsal boundary of the circling substrate was located within the pedunculus cerebellaris superior or the floor of the substantia grisea centralis. In addition, the positive region extended 1.7–2 mm ventrally and 1.9 mm from the midline. The distribution of the positive sites seems to suggest that the circling substrate is a large bundle which runs sagittally through the medial part of the reticular formation.