Abstract
Ethanol-precipitated red wine polysaccharides were fractionated by a combination of anion-exchange, size-exclusion and affinity chromatography steps. This comprehensive fractionation allowed us to prepare a collection of wine polysaccharides in sufficient amount to permit the determination of their intrinsic properties. Glycosyl-residue composition of each polysaccharide fraction was determined by GC–EI–MS of the per- O-trimethylsilylated methyl glycoside derivatives (TMS), a method that has been recently developed and adapted to suit simultaneous determination of neutral and acidic glycosyl-residue compositions of polysaccharides present in plant-derived products. The results showed that mannoproteins released by yeast during fermentation, and grape derived arabinogalactan-proteins, rhamnogalacturonans I and II are the main wine polysaccharides and accounted for 35, 42, 4 and 19%, respectively, of the total polysaccharides. Structural characterization revealed that rhamnogalacturonan I fractions were linked with xyloglucan-like polysaccharides. This finding represents compelling evidence of the existence of cross-linking between pectin and hemicellulose domains in plant primary cell walls.
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