The Polymorphisms of PPARγ Pro12Ala and PGC-1 Gly482Ser in Chinese Women With Polycystic Ovary Syndrome

Abstract

ObjectivePolycystic ovary syndrome (PCOS) is one of the most common endocrine metabolic diseases and is characterized by ovarian androgen overproduction, polycystic ovary and oligo- or anovulation. Insulin resistance has been implicated in the pathogenesis of ovarian dysfunction in PCOS. Insulin-mediated carbohydrate metabolism and sensitizing effects of thiazolidinedione are regulated at least in part by peroxisome proliferator-activated receptor-γ (PPARγ) and peroxisome proliferator activated receptor γ coactivator-1 (PGC-1).In this study, we defined the role of polymorphisms of PPARγ Pro12Ala and PGC-1 Gly482Ser in Chinese women with Polycystic ovary syndrome.DesignControlled clinical study .Materials and methodsTwo hundred and one PCOS patients and 147 controls were recruited in University-affiliated department. Menarche age was recorded. Body mass indices were calculated. Blood samples were obtained for DNA analyses of PPARγ and PGC-1 polymorphisms,and hormone measurements.ResultsIn Chinese subjects the frequencies of genotypes were as follows: 0.914 Pro12pro, 0.083 Pro12Ala, 0.003 Ala12Ala for the PPARγ2 gene and 0.329 Gly482Gly, 0.392 Gly482Ser, 0.279 Ser482Ser for the PGC-1 gene. The Ala allele frequency (4.4%) is lower, and Ser allele frequency (34.1%) is higher in Chinese subjects, as compared with that of western countries. Both Pro12Ala polymorphism and Gly482Ser polymorphism distributions are similar between Chinese women with PCOS and control. There are no significant differernces of BMI and reproductive hormones such as Total testosterone (T) levels, FSH, LH, P, E2 and PRL levels between the patients and controls.ConclusionOur data suggests there is no association between these polymorphisms and the risk of PCOS in Chinese subjects. ObjectivePolycystic ovary syndrome (PCOS) is one of the most common endocrine metabolic diseases and is characterized by ovarian androgen overproduction, polycystic ovary and oligo- or anovulation. Insulin resistance has been implicated in the pathogenesis of ovarian dysfunction in PCOS. Insulin-mediated carbohydrate metabolism and sensitizing effects of thiazolidinedione are regulated at least in part by peroxisome proliferator-activated receptor-γ (PPARγ) and peroxisome proliferator activated receptor γ coactivator-1 (PGC-1).In this study, we defined the role of polymorphisms of PPARγ Pro12Ala and PGC-1 Gly482Ser in Chinese women with Polycystic ovary syndrome. Polycystic ovary syndrome (PCOS) is one of the most common endocrine metabolic diseases and is characterized by ovarian androgen overproduction, polycystic ovary and oligo- or anovulation. Insulin resistance has been implicated in the pathogenesis of ovarian dysfunction in PCOS. Insulin-mediated carbohydrate metabolism and sensitizing effects of thiazolidinedione are regulated at least in part by peroxisome proliferator-activated receptor-γ (PPARγ) and peroxisome proliferator activated receptor γ coactivator-1 (PGC-1).In this study, we defined the role of polymorphisms of PPARγ Pro12Ala and PGC-1 Gly482Ser in Chinese women with Polycystic ovary syndrome. DesignControlled clinical study . Controlled clinical study . Materials and methodsTwo hundred and one PCOS patients and 147 controls were recruited in University-affiliated department. Menarche age was recorded. Body mass indices were calculated. Blood samples were obtained for DNA analyses of PPARγ and PGC-1 polymorphisms,and hormone measurements. Two hundred and one PCOS patients and 147 controls were recruited in University-affiliated department. Menarche age was recorded. Body mass indices were calculated. Blood samples were obtained for DNA analyses of PPARγ and PGC-1 polymorphisms,and hormone measurements. ResultsIn Chinese subjects the frequencies of genotypes were as follows: 0.914 Pro12pro, 0.083 Pro12Ala, 0.003 Ala12Ala for the PPARγ2 gene and 0.329 Gly482Gly, 0.392 Gly482Ser, 0.279 Ser482Ser for the PGC-1 gene. The Ala allele frequency (4.4%) is lower, and Ser allele frequency (34.1%) is higher in Chinese subjects, as compared with that of western countries. Both Pro12Ala polymorphism and Gly482Ser polymorphism distributions are similar between Chinese women with PCOS and control. There are no significant differernces of BMI and reproductive hormones such as Total testosterone (T) levels, FSH, LH, P, E2 and PRL levels between the patients and controls. In Chinese subjects the frequencies of genotypes were as follows: 0.914 Pro12pro, 0.083 Pro12Ala, 0.003 Ala12Ala for the PPARγ2 gene and 0.329 Gly482Gly, 0.392 Gly482Ser, 0.279 Ser482Ser for the PGC-1 gene. The Ala allele frequency (4.4%) is lower, and Ser allele frequency (34.1%) is higher in Chinese subjects, as compared with that of western countries. Both Pro12Ala polymorphism and Gly482Ser polymorphism distributions are similar between Chinese women with PCOS and control. There are no significant differernces of BMI and reproductive hormones such as Total testosterone (T) levels, FSH, LH, P, E2 and PRL levels between the patients and controls. ConclusionOur data suggests there is no association between these polymorphisms and the risk of PCOS in Chinese subjects. Our data suggests there is no association between these polymorphisms and the risk of PCOS in Chinese subjects.