The polymorphic region an interleukin B1 is highly associated with type 1 diabetes in Saudis

Abstract

Type 1 diabetes (T1D) is an auto‐immune disease mediated by T lymphocytes that lead to insulin secreting islet B cell destruction. T1D is a multifactorial disease with strong genetic component. Pancreatic sample from patients with T1D showed mononuclear cell infiltration in islets, include mainly B cells and T cells that required for disease development.In this study, we genotyped a group of Single Nucleotide Polymorphisms (SNPs) that located at IL1B1 (rs16944), IL1A (rs1800587), IL1B (rs1143634), IL1R (rs2234650) and IL1RN (rs315952) cytokines and cytokine receptors to investigate a possible association between these genetic markers and T1D.Sequence‐specific forward and reverse primers and two TaqMan® MGB probes with dyes (VIC/FAM) were used to detect allele 1 and allele 2 of each SNP. 150 patients with T1D and 150 normal healthy individuals with no history of auto‐immune disease were recruited. The genotype models for each SNP (codominant, dominant and recessive traits) were derived using the SNPStat software, Fisher exact test and logistic regression methods were used to compare patient and control results. The dominant model of rs16944 (G/G vs G/A‐A/A)) was found highly significant in patient than in control (41.3% vs 14.6%, p value <0.0001) and considered as a risk factor for T1D development and individuals having this genotype are 4 fold susceptible to develop the disease. No other significant association was detected.In conclusion, rs16944G/G model is considered positively associated with T1D.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.