Abstract
Liver cells were isolated by a collagenase perfusion technique. A fraction of the cell suspension containing mostly parenchymal cells was obtained by slow speed centrifugation. Separated, intact cells were stained with mitramycin for flow cytometry. A brief pre-treatment with toluene was found necessary for rapid and uniform staining. Isolated nuclei were prepared by a combination of detergent and pepsin treatments and stained with ethidium bromide.
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