The Plastoglobule‐localized AtABC1K6 is a Mn2+‐dependent Protein Kinase Necessary for Timely Transition to Reproductive Growth

Abstract

The Absence of bc1 Complex (ABC1) is an ancient, atypical protein kinase family that emerged prior to the archaeal‐eubacterial divergence. Loss‐of‐function mutants in ABC1 genes are linked to respiratory defects in microbes and humans, and to compromised photosynthetic performance and stress tolerance in plants. However, demonstration of protein kinase activity remains elusive, hampering their study. Here, we investigate a homolog from Arabidopsis thaliana, AtABC1K6, and demonstrate in vitro protein kinase activity as autophosphorylation, which we replicate with a human ABC1 ortholog. We show that AtABC1K6 protein kinase activity requires an atypical buffer composition, including Mn2+ as divalent cation co‐factor and a low salt concentration. AtABC1K6 associates with plastoglobule lipid droplets of A. thaliana chloroplasts, along with five paralogs. Protein kinase activity associated with isolated A. thaliana plastoglobules was inhibited at higher salt concentrations, but could accommodate Mg2+ as well as Mn2+,indicatingsalt sensitivity, but not the requirement for Mn2+, may be a general characteristic of ABC1s. Loss of functional AtABC1K6 impairs the developmental transition from vegetative to reproductive growth. This phenotype is complemented by the wild‐type sequence of AtABC1K6 but not by a kinase‐dead point mutant in the unique Ala‐triad of the ATP‐binding pocket, demonstrating the physiological relevance of the protein’s kinase activity. We suggest that ABC1s are bona fide protein kinases with a unique regulatory mechanism. Our results open the door to detailed functional and mechanistic studies of ABC1s and plastoglobules.