Abstract
Plasma membrane Ca 2+-ATPase is involved in the fine-tuned regulation of intracellular Ca 2+. In this study, the presence of Ca 2+-ATPase in caveolae from kidney basolateral membranes was investigated. With the use of a discontinuous sucrose gradient, we show that Ca 2+-ATPase is exclusively located and fully active in caveolin-containing microdomains. Treatment with methyl-β-cyclodextrin – a cholesterol chelator – leads to a spreading of both caveolin and completely inactive Ca 2+-ATPase toward high-density fractions. These data support the view that Ca 2+ fluxes mediated by Ca 2+-ATPase in kidney epithelial cells occur only in caveolae, being strictly dependent on the integrity of these microdomains.
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