The pinealocyte forming receptor and effector endings: Immunoelectron microscopy and calcium histochemistry.

Abstract

The pinealocytes--the main cellular elements of the pineal organ--are polarized, displaying a (photo)receptor and an axonic effector cell pole. The receptor endings are of two main types: they bear rod-type or cone-type outer segments characterized by the presence of immunoreactive opsin-, S-antigen- and vitamin A-binding sites. The effector pole may form ribbon-containing synapses on the secondary pineal neurons, and/or neurohormonal terminals on the basal lamina of the pineal nervous tissue. Applying potassium pyroantimonate (PPA) to electron-microscopic histochemistry, we found in the frog that both effector terminals and photoreceptor outer segments contained a large amount of Ca-pyroantimonate deposit similar to retinal cones and rods. Rods and rod-like pinealocytes contained more deposits than cones. The higher concentration of calcium on the cell membranes of dark pinealocytes in the rat may be connected with their rod-like character. In the frog, a high amount of calcium seemed to be concentrated in the photoreceptor effector terminals, especially around their synaptic ribbons, and in myeloid bodies of the pineal ependyma and retinal pigment epithelium. Calcium was richly found in or around corpora arenacea in the human and rat pineal. It is suggested that the formation of concrements may be connected with the high demand of Ca-exchange of pinealocytes for their receptor and effector membrane functions. In the rat, lymphocytes were found to migrate through the wall of the vena magna of Galen and to closely contact pinealocytes, presumably to receive immunological information as an additional pineal output.