Abstract

By polarographic determination of the dissolved oxygen concentration of media used to flood eggs of Aedes aegypti and A. nigromaculis, the effect of dissolved oxygen in stimulating hatching was found to be twofold. An inverse relation occurred between dissolved oxygen and hatching of eggs flooded under conditions of static oxygen level, very low levels being required to induce a high degree of hatching. In contrast, even a slight lowering of the oxygen level while eggs were flooded proved a powerful hatching stimulus. A decline lasting several hours was found in the dissolved oxygen level at the soil-water interface of flooded soil, so that a decreasing level, rather merely a very low level, of dissolved oxygen seems to represent the natural mode of stimulation. Exposure of eggs to 50% relative humidity significantly reduced the hatching response as compared with that of eggs kept under high-humidity conditions.

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