The phylogeny of Mycoplasma bovis from pneumonic goats based on16S rRNA Sequences in El-Salam Canal at northern Sinai Governorate

Abstract

Mycoplasma bovis (Mb) is significantruminant respiratory pathogen of economic importance. This study determined the incidence of isolation of Mb in association with pneumonia based on respiratory manifestation in goats and examined the antigenic variability between same species of Mb in El-Salam Canal at northern Sinai Governorate. Three hundred and thirty five samples were collected from goats suffering from respiratory manifestations (274 nasal swabs and 61 lung tissue). The samples were subjected to Mycoplasma (M) and Mb isolation by culture and polymerase chain reaction (PCR) based on 16S rRNA gene specific primers. Mb was recorded in 8% and 9.8% and 11.4% and16.4% from nasal swabsand pneumonic lungs to the total no. of samples by culture and PCR respectively. Results revealed that, Mb. is one of the important pathogen causing pneumonia in goats. In additions, there was great dissimilarity of 16S rRNA gene between isolated strains and the isolates was harboring a novel genotype. Histopathological findings supported the mycoplasmic lesions. This research provides the first evidence of isolation and identification of Mb from the nasal swabs and lungs of goats with respiratory problems. The study showed for the first time the sequence analysis of 16S rRNA gene of this pathogen isolated from pneumonic goats in this area.