The phylogeny of monkey beetles based on mitochondrial and ribosomal RNA genes (Coleoptera: Scarabaeidae: Hopliini)

Abstract

Monkey beetles (Hopliini) are a large clade of flower and leaf feeding species within the Scarabaeidae (chafers) with greatest diversity in southern Africa. Their internal relationships and sister group affinities have not been studied with DNA methods. We used partial gene sequences for 28S rRNA, cytochrome oxidase I (cox1) and 16S rRNA (rrnL) for 158 species, representing most recognized subfamilies of Scarabaeidae, including 46 species of Hopliini. Combined analyses using maximum likelihood and Bayesian inference under the two preferred alignment parameters recovered the Hopliini as monophyletic. Hopliines were inserted at the base of a clade of Cetoniinae+Rutelinae+Dynastinae, being either recovered as their immediate sister group, or as part of an expanded set of basal branches that also includes the tribe Macrodactylini which has been classified as part of the Melolonthinae (may chafers). At the level of subtribes, we found Hopliina paraphyletic with respect to Pachycnemina which also includes the monophyletic clade of Heterochelina and Gymnolomina. Trait mapping under parsimony on the preferred tree resulted in inferences of three independent origins of sexual dimorphism, which coincided with shifts to ‘flower-embedding’ pollination. In contrast, night active taxa, which are general phyllophages as other pleurostict chafers, never show clear sexual dimorphism. South African lineages include several deep-branching lineages. The exceptional morphological and phylogenetic diversity of the South African fauna may therefore be due to their antiquity, in addition to sexual selection in the day-active lineages. Phylogenetic studies of the endemic South African plant radiations have demonstrated the repeated evolutionary shift to beetle pollination, but it remains to be investigated if this is driven by the hopliine pollinators present in the bioregion or by a propensity of the local plant lineages favoring this pollination syndrome.