The phylogenetic relationships of the q6-equipped generatorulaspora lindner and zygosaccharomyces barker (saccharomycetaceae) based on the partial sequences of 18s and 26s ribosomal ribonucleic acids.

Abstract

Eleven strains of Torulaspora and Zygosaccharomyces species were examined for the partial base sequences of 18S and 26S rRNAs. In the partial base sequences in positions 493-622 (130 bases) of 26S rRNA, there were 88-91% and 70-92% maximum homologies within the genera Torulaspora and Zygosaccharomyces, respectively. The maximum homologies were 70-90% between the two genera. The species of the two genera had 73-88% maximum homologies with Saccharomyces cerevisiae. In the partial base sequences in positions 1611-1835 (225 bases) of 26S rRNA, there were 3-0 and 16-1 base differences within the two genera, respectively. The base differences were 15-0 between the two genera. The two genera had 17-8 base differences with S. cerevisiae. In the partial base sequences in positions 1451-1618 (168 bases) of 18S rRNA, there were 0 and 3-0 base differences within the two genera, respectively. The base differences were 2-0 between the two genera. The two genera had 3-1 base differences with S. cerevisiae. Zygosaccharomyces cidri and Z. fermentati occupied a unique situation (base differences, 3-2). The data obtained were discussed with regard to the phylogenetic relationships and the taxonomic positions of Torulaspora, Zygosaccharomyces, and Saccharomyces.