Abstract
Abstract— The photoreaction of the artificial pigment synthesized from bacterioopsin and rrans‐3,4‐dehydro‐retinal, [3,4‐dehydro]bacteriorhodopsin ([3,4‐deH]bRt) was investigated with low temperature spectrophotometry.The amount of batho‐product formed from the light‐adapted pigment of [3,4‐deH]bR (designated as batho‐[3,4‐deH]bRt) by irradiation at 77 K was much less than that from trans‐bacteriorhodopsin (bRt) and depends on temperature at irradiation of the sample. The kinetics of photoconversion of [3,4‐deH]bRt to batho‐[3,4‐deH]bRt and that of its reversion were measured at several temperatures with a so‐called “double Dewar system”. The results showed that the photosensitivity of [3,4‐deH]bRt was temperature dependent. When batho‐[3,4‐deH]bRt was warmed above 143 K, it was converted to lumi‐[3,4‐deH]bRt. Lumi‐[3,4‐deH]bRt was also produced by irradiation of [3,4‐deH]bRt at 143 or 163 K. The maximum in the difference spectrum between lumi‐[3,4‐deH]bRt and [3,4‐deH]bRt was located at about 540 nm. The irradiation of [3,4‐deH]bRt at 183 K produced an intermediate analogous to meta‐bRt, but under similar conditions bRt does not produce meta‐bRt. These results indicate that intermediates of [3,4‐deH]bRt are less stable than those of bRt.The differences between bacteriorhodopsin and [3,4‐dehydro]bacteriorhodopsin were discussed and compared with the differences between rhodopsin and [3,4‐dehydro]rhodopsin.
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