The photoprotective effect of 1,25-dihydroxyvitamin D 3 on ultraviolet light B-induced damage in keratinocyte and its mechanism of action

Abstract

We investigated the photoprotective effect of 1,25-dihydroxyvitamin D 3 (1,25(OH) 2D 3) both in vivo and in vitro, revealing its relationship with glutathione, a well-known antioxidant. We also probed into the possible mechanism of photoprotection of 1,25(OH) 2D 3 through immunohistochemical study for metallothionein (MT). At the same time, endogenous antioxidant effect of 1,25(OH) 2D 3 was examined. Survival of cultured human keratinocytes was decreased when the cells were irradiated with ultraviolet light-B (UVB) at doses above 30 mJ/cm 2. But in the presence of 1,25(OH) 2D 3 (12 nM), the decrease of survival of keratinocytes by UVB was diminished. The formation of sunburn cells by UVB irradiation in the skin of ICR mice was inhibited by topical application of 1,25(OH) 2D 3, regardless of prior glutathione depletion. Immunohistochemical staining revealed that 1,25(OH) 2D 3 induced the expression of MT, a potent radical scavenger, mainly in the basal layer of ICR mice skin. 1,25(OH) 2D 3 neither inhibited peroxidation of plasma lipids nor interacted with superoxide, nor removed hydrogen peroxide as an antioxidant. These findings suggest that 1,25(OH) 2D 3 has photoprotective effect not related with glutathione or its endogenous antioxidant property. Rather, it could be attributed to 1,25(OH) 2D 3-induced MT and its capacity to prevent radical-related damage in UVB irradiation.