The phenotypic and genetic characterization of some virulence factors in MRSA isolated from burn patients

Abstract

The present study aimed to the phenotypic and genetic detection of some virulence factors in Methicillin resistant Staphylococcus aureus (MRSA) isolated from burn patients. from a total of 38 isolates of Staphylococcus aureus, 15(39.4%) isolates of MRSA were collected from the patients in Diwaniyah Teaching Hospital during the period from June to October, 2018 in AL-Diwaniyah City. Methicillin resistant Staphylococcus aureus (MRSA) was detected by using chrom agar MRSA and polymerase chain reaction (PCR), 15 isolates (39.4%) reported resistance to methicillin antibiotic.In addition, phenotypic detection of the ability of MRSA to produce the ß-lactamase enzymes using rapid iodometric method and its ability to form the biofilm was determined by using the tube method, The results showed 15(100%) MRSA isolates were able to produce ß-lactamase enzymes and 14 (93.3%) MRSA isolates were able to biofilm production. On the other hand, All MRSA isolates were tested by using Polymerase Chain Reaction (PCR) to detection of some virulence factors genes, which include coa gene encoding production of coagulase, icaA gene encoding for biofilm production and blaZ gene encoding for the ß-lactamase enzymes. PCR results showed that MRSA isolates were posses blaZ gene (100%), icaA gene (93.3%) and coa gene (86.6%).