Abstract
This study investigated the antioxidative and obsteoblast differentiation promoting activity of the phenolics isolated from the 70% ethanol extract of the roots of Livistona chinensis. Two new phenolics, (2R,3R)-3,5,6,7,3',4'-hexahydroxyflavane (1), and phenanthrene-2,4,9-triol (2), together with six known phenolics 3–8, were isolated and identified on the basis of extensive spectroscopic analysis. The antioxidative and obsteoblast differentiation promoting abilities of the compounds 1–3, 7–8 were tested, the phenolics 1–3, 7 showed effects on proliferation of osteoblastic cells and antioxidative activity of 3.125–50 µg/mL. In addition, the phenolics 1–3 observably increased alkaline phosphatase activity, osteocalcin content and hydroxyproline content in osteoblastic cells. Phenolic 1 at 12.5 µg/mL concentration significantly increased the area of nodules by about 9.35-fold. The antioxidative activity results indicated that the anti-osteoporosis effects of these phenolics may be linked to a reduction of oxidative stress. The observed effects of these phenolics on bone formation by rat osteoblastic cells suggest that these phenolics may have beneficial effects on bone health.
Highlights
Osteoporosis is a disease characterized by the loss of bone mass and degeneration of bone microstructure, resulting in an increased risk of fractures
As part of our ongoing search for active anti-osteoporosis compounds from traditional medicinal plants, we investigated the constituents from the roots of L. chinensis
To clarify the underlying mechanisms of action of the phenolics, we investigated whether protection against osteoporosis was linked to a reduction of oxidative stress
Summary
Osteoporosis is a disease characterized by the loss of bone mass and degeneration of bone microstructure, resulting in an increased risk of fractures. Osteoporosis, called postmenopausal osteoporosis, is common in women after menopause [1] It may develop in men, especially in the aged man, which is called age-related bone loss [2]. Oxidative stress, resulting from excessive formation of reactive oxygen species (ROS) or dysfunction of antioxidant defense system, represents a major cause of age-associated pathological conditions including aging [3] and postmenopausal bone loss [4]. Oxidative stress is a pivotal pathogenic factor for age-related bone loss in mice, leading to an increase in osteoblast and osteocyte apoptosis and a decrease in osteoblast number and the rate of bone formation [5,6]. To clarify the underlying mechanisms of action of the phenolics, we investigated whether protection against osteoporosis was linked to a reduction of oxidative stress
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