Abstract

Silver fir (Abies alba Mill.) is one of the most common and valuable conifer tree species in Central Europe, with well-established usage in the construction and furniture industries, as well as the food, health products, pharmaceuticals and cosmetics industries. Silver fir branch extract, a mixture of antioxidative phenols, is produced industrially as a food supplement with a wide range of therapeutic properties. This study investigates optimization of the production of silver fir branch extract by researching its antioxidant activity (ABTS and DPPH assay), phenol content (Folin-Ciocalteu assay), lignan content (HPLC) and extractable content at various distances from the trunk (0–80 cm). The antioxidative activity, phenol content and extractable content decreased from the proximal to the distal part of the branch. The decrease in ABTS assay activity was 51%, and that of the DPPH assay was 52%; the decrease in total phenol content was 35–40%; and the decrease in lignan content was 91%. The extractable matter content was reduced by 40%. Data gained in the study herein justifies the importance of researching existing and industrially produced plant extracts for further optimization of the final product. Results shows that industry can also produce extracts with elevated content of lignans with the use of short proximal parts of the branches.

Highlights

  • Academic Editors: Simon Vlad Luca, Silver fir (Abies alba Mill.) is one of the most common and valuable conifer tree species in Central Europe for historical and economic reasons [1]

  • ABTS (2,20 -azinobis-(3-ethylbenzthiazoline-6-sulfonic acid)) (CAS: 30931-67-0) and DPPH (2,2-diphenyl-1-picrylhydrazyl) (CAS: 1898-66-4) were purchased from SigmaAldrich® (Darmstadt, Germany); gallic acid, potassium peroxodisulfate and Na2 CO3 were purchased from Fluka (Darmstadt, Germany); methanol was purchased from Carlo Erba (Milan, Italy); and Folin–Ciocalteu (FC) reagent was purchased from Merck (Darmstadt, Germany)

  • The solvents used for the high-performance liquid chromatography (HPLC)

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Summary

Introduction

Academic Editors: Simon Vlad Luca, Silver fir (Abies alba Mill.) is one of the most common and valuable conifer tree species in Central Europe for historical and economic reasons [1]. Its usage for consumption started over a century ago in the production of, for example, silver fir beer [2]. It is a medicinal plant that is used in the food, health products, pharmaceuticals and cosmetics industries [3,4,5]. Research and publications provide scientific evidence to support claims that silver fir phytochemicals provide health benefits to consumers. One of the largest groups of phytochemicals comprises phenolic chemicals, which are universally present in higher plants [6]. Polyphenols could improve insulin resistance [11] and be used as radioprotective agents [12]

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