Abstract
A total of 55 phenolic constituents was extracted with methanol – 1 % HCl at room temperature from dried leaves of cassava (Manihot esculenta Crantz). By means of thin-layer chromatographic and spectrophotometric techniques the 20 main phenolic constituents were identified as quercetin and luteolin glycosides, chlorogenic acid, esters of p-coumaric, caffeic, ferulic, and sinapic acids, and the glycosides of caffeic and ferulic acids. Benzoic acid derivatives were also confirmed. Acid and alkaline hydrolysis of the major compounds was carried out to determine their aglycones. Ultraviolet (UV) spectral data, Rf values, fluorescence in UV light, and color reactions with chromogenic spray reagents of the phenolic compounds and the aglycones are presented. The sugar residues of the major flavonoid compounds were identified as glucose.
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