Abstract
The synthetic poly-amino acid, poly-l-lysine has been covalently coupled to a ruthenium polypyridyl complex, a long-lived fluorescent species, via a thiourea linkage between the amino side groups of the lysine residues and the isothiocyanate group of the label. The pH dependence of the emission lifetime of the label is reported whereby the behavior observed is explained in terms of the conformational changes of the bound biomolecules due to the label's sensitivity to its local environment.
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