Abstract
Ethidium bromide is known to convert respiratory sufficient yeast (grandes) to cytoplasmically inherited respiratory deficient mutants (petites) at efficiencies close to 100%. We have investigated the mechanism of this conversion in Saccharomyces cerevisiae by examining the properties of mitochondrial DNA at various times during the mutation process, using cycloheximide to amplify the proportion of radioactivity in mitochondrial DNA. Ethidium bromide is shown to inhibit selectively mitochondrial DNA synthesis; furthermore, in its presence, pre-existing mitochondrial DNA is progressively degraded. In petites made by prolonged treatment with ethidium bromide no mitochondrial DNA could be detected.
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